ANTIMICROBIAL, ANTIOXIDENT AND CYTOTOXIC EVALUATION OF IFLOGA SPICATA (Forssk) Sch.Bip. BIOSYNTHESIZED SILVER NANOPARTICLES

Abstract

The study aimed to determine pharmacological and phytochemical potential of different solvent fractions of Ifloga spicata. Antimicrobial potential, free radical scavenging activity 1, 1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide scavenging assay, and cytotoxic effects of plant samples on human blood lymphocytes under H₂O₂ induced stress was performed. Ifloga spicata ethyl acetate fraction markedly showed higher antibacterial potential against P. aeruginosa (ATCC 27853) and E.coli (ATCC 25922) bacterias. While higher antifungal potential was shown by the chloroform fraction against all three different strains A. fumigates (ATCC 204305), A. flavus (ATCC 9643) and A. niger (ATCC 16404) as compared to others fractions of the sample. DPPH maximum antioxidant potential was noted for methanolic extract followed by ethyl acetate with order of Methanol>Ethyl acetate>Chloroform>n-Hexane. Similarly, the hydrogen peroxide effect was significantly decreased by ethyl acetate fraction. In blood lymphocytes the raise in ROS and TBARS level due to stress given by H₂O₂ was also recovered by different extracts of I. spicata. The decrease level of  superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) was also restored to its normal by all extract of the Ifloga Spicata.