DETERMINATION OF ANTI-ARTHRITIC ACTIVITY OF THE LEAVES EXTRACTS OF PROSOPIS JULIFLORA (SW) DC BY USING IN VITRO METHOD

Abstract

Rheumatoid arthritis (RA) is an autoimmune inflammatory condition with an unknown aetiology that causes cartilage and bone to erode. The ineffectiveness and side effects of the current therapeutic agents, such as glucocorticoids, disease-modifying anti-rheumatic drugs, and non-steroidal anti-inflammatory drugs (NSAIDs), highlight the need for finding new agents with more significant therapeutic potential and fewer side effects. The plant Prosopis Juliflora, also known as mesquite and a member of the Fabaceae family, has been used as a general remedy for a variety of ailments, including catarrh, diarrhoea, dysentery, wound healing, flu, measles, hoarseness, infection, and sore throat. The anti-arthritic effect of P. juliflora leaves of plant was subjected to four extraction methods: maceration, sonication, soxhalation and reflux extraction. In vitro study was conducted by using a protein denaturation assay. The IC50 values of macerated, PJE, PJE50 and PJA extracts were 355.02, 302.8, and 230.1 ?g/mL, respectively. The IC50 values of the sonicated crude extracts, i.e., PJE, PJE50 and PJA, were calculated as; 3792.2, 1129.4 & 44.91 ?g/mL, respectively. The IC50 values of refluxed crude extracts PJE, PJE50 and PJA were calculated as; 250.35, 337.80 & 130.36 ?g/mL, respectively. The IC50 value of soxhlated crude extract PJE was determined as 160.50 ?g/mL. The result shows that all the extracts possessed protein denaturation-inhibiting activity. Among all crude extracts, the PJA extract made by the sonication method is the most potent with the lowest IC50 value.